You will write a report describing this enzyme: EC 1.1.3.4 of Aspergillus niger

Instructions
Perform a literature search to find out all that you can about this enzyme. These are the properties of the enzyme that you will need to cover in your assignment:

·         Ø  EC nomenclature, systematic name, nickname, and species from which it was isolated

·         Ø  Reaction catalyzed (show structures)

·         Ø  Number of amino acids in the sequence, molecular weight, oligomeric state

·         Ø  Cofactor used (if any), and its role in catalysis

·         Ø  Optimum pH and temperature

·         Ø  Kinetic parameters (KM and kcat), for natural substrates

·         Ø  Substrate specificity (i.e. list natural substrates known)

·         Ø  Catalytic residue(s) and type(s) of catalysis (other than “proximity and orientation catalysis” and “preferential transition state binding catalysis”)

·         Ø  Biological role

·         Ø  Assay used to measure kinetic parameters (show reaction)

·         Ø  Inhibitor and type of inhibition

·         Ø  Mechanism (show reactions), two key experiments that were used to study mechanism, Cleland diagram, Cleland nomenclature for mechanism

-       Write a report summarizing the findings from your literature review. Use the provided template to do this.

-       Your report needs to cite a minimum of 5 research articles (not reviews) in which the enzyme's properties are studied. Make sure that you read these articles in detail and cite them as you would in a research article. Additionally, deriving all your content from the introduction section of articles will not be accepted.

Requirements

·         Ø  Use the template provided

·         Ø  A minimum of 5 cited research articles, not reviews.  

·         Ø  Append PDF files for all references used to make figures for your assignment.

·         Ø  If you think it useful, you can add text to describe in more detail any of the enzyme properties. Write in a clear style, i.e. you will lose points for grammar, syntax and orthographic errors. Be concise, clear, and specific.

·         Ø  Please ensure that your figures are not pixelated, and that the legends are clear.

·         Ø  DO NOT PLAGIARIZE FIGURE LEGENDS! Write them in your own words. It’s however OK to copy the image itself, as long as you state so and provide reference.

Ø For finding general information on enzymes: www.brenda-enzymes.org, www.chem.qmul.ac.uk/iubmb/enzyme

Ø For searching research articles: www.ncbi.nlm.nih.gov/pubmed Ø For finding the structure of the enzyme: www.rcsb.org

-       AN EXAMPLE IS PROVIDED ON PAGE 2-9, the template from pages 10-17