Watch and discuss the videos as a group, then answer the related questions. This assignment will be graded, and is due by 5pm on Tuesday, October 13th.
science
Description
Instructions:
Watch and discuss the videos as a group,
then answer the related questions. This
assignment will be graded, and is due by 5pm on Tuesday, October 13th. The assignment should be submitted to the
Assignments section of the lecture Webcampus page. You may turn the assignment in as a group, as
part of your group, or individually.
Please be sure all names of the people who are to be given the same
grade for the assignment are typed on the top of the assignment. You will be submitting your answers to
SafeAssign, so please use your own words.
The
document you submit to Safe Assign should only have the question numbers and
your answers in it. Please delete the
instructions, video links and questions themselves so that they do not show up
as matches to other classmates’ assignments in SafeAssign.
In this lab, you will learn about a few ways
in which DNA is analyzed in order to determine matches between DNA
samples. We will mainly focus on
analysis used in forensic DNA fingerprinting and relatedness between
individuals.
DNA sequencing can be performed using Sanger
sequencing (although newer methods like next generation sequencing are now
being used). Sanger sequencing is a
modified version of DNA replication. Please
watch the following video that provides an overview of the Sanger sequencing
method:
·
https://www.youtube.com/watch?v=e2G5zx-OJIw
DNA can also be analyzed by looking at short
tandem repeats (STR’s). Please watch the
following two videos that provide an overview of the STR analysis:
·
https://www.youtube.com/watch?v=7onjVBsQwQ8
·
https://www.youtube.com/watch?v=-RzKFR-n5zs
1)
What is the goal or product of Sanger
sequencing?
2)
What is the goal or product of STR
analysis?
3)
What is one major way in which the
methods of Sanger sequencing and STR analysis are similar?
4)
What is one major way in which the
methods of Sanger sequencing and STR analysis are different?
STR analysis is performed to match DNA samples
collected from different sources (such as from a crime scene and from
suspects). Please watch the following
two videos that provide more information about forensic DNA analysis:
·
https://www.youtube.com/watch?v=kF6EGa8GpD0
·
https://www.youtube.com/watch?v=HdzBh6sMLA0
5)
Why is STR analysis done to compare
DNA samples (crime scene and suspects) and not DNA sequencing?
On the right
is a readout of several STR’s from DNA collected at a crime scene. Below the
image is the DNA data from a suspect.
The first instance of the repeating unit is underlined for each locus.
